Principal Investigator: Kenneth P. Wright Jr., Ph.D. Date: 5/16/21

This study is conducted by the Sleep and Chronobiology Laboratory at the University of Colorado at Boulder.


Morning Shift Work Microbiome Study Requirements: Healthy Men & Women Age 18-35


This study is about how early morning work shifts change molecules in your blood, including your blood sugar levels, and alters the bacteria that live in your gut and typically help keep you healthy. We are testing how a prebiotic diet alters how you and the bacteria living in your gut respond to you working early morning shifts. This work will have important implications for the development of treatments and countermeasures for people who do not sleep enough and need to be work in the early morning hours (e.g., emergency and health care workers, military and transportation personnel, service professionals) and other morning shift workers. This research study in early morning shift-workers is part of a larger study examining how a prebiotic diet may alter the gut bacteria and molecules in blood when experiencing sleep restriction and circadian misalignment (being awake when you are supposed to be sleeping).

Prior to the start of this study, there are screening procedures to ensure you meet the criteria for the study. At your first consent screening appointment, you will learn more about the study, answer questionnaires, and complete a psychological interview. At your second medical screening appointment, you will have a physical exam, blood work, drug screen, pregnancy test, electrocardiogram (a measurement of heart activity. Although you are not financially compensated for the screening procedures, we do cover the expense of the screening tests. All of the results of the screening procedures are confidential and will only be used by the study staff to determine eligibility for the study.


Following the screening procedures, you will complete a study which lasts approximately 5 weeks and is mostly done at home. During your home monitoring, we ask you to wear a special watch that records your activity levels. We also ask you to keep a sleep/wake log and use a website to log your daily sleep/wake times (if you cannot access this website, you can call into our answering machine to inform us when you go to bed and wake up each day). For a total of 4 weeks, you will also be provided a powdered prebiotic or maltodextrin placebo that we will ask you to consume once daily, mixed with water. Throughout the study you will be given both a prebiotic and a placebo, but the order of treatment is randomized. It is possible you may receive the prebiotic first and will consume that for two weeks until receiving the placebo second for two weeks. Otherwise you would receive the placebo first for two weeks and will consume that daily until receiving the prebiotic second for two weeks. There will be one week period between the prebiotic and placebo conditions with no supplement or monitoring.


During the home monitoring, you will be outfitted with a Continuous Glucose Monitor which will be placed on the back of your arm, which takes your glucose levels periodically for 14 days at a time. You will have 2 continuous glucose monitors in total. Additionally, for 7 nights on two different occasions (14 nights total), you will wear a Dreem ambulatory PSG headband which measures your sleep at home. You will also have one night at home where you wear a breathing monitor to sleep to determine if you may be at risk for a sleep disorder called sleep apnea.
We will take blood samples on two occasions at our lab to test for immune and metabolic markers. We will also ask you to collect samples of the bacteria that live in your gastrointestinal tract from used bathroom tissue after you use the bathroom on certain days at home during the 4 week study. There are a total of 8 visits that you would need to complete if you are interested in participating in this study, including 2 screening visits and 6 study visits.


If you are eligible to participate in this study, you will be financially compensated for your involvement after the screening procedures. You will be compensated $75 for each week of home activity monitoring and $25 for each week of Continuous Glucose Monitoring. The total compensation can be up to $400.


If you are interested in applying to participate in this study, please complete this on-line application survey. We will contact you within one week to let you know if you qualify for the first consent screening appointment. If you need assistance with this survey, have additional questions about the study, or would like to complete the survey via the telephone, please contact us at (303) 735-1923 (M-F, 9am-5pm). With the email address of sleep.study@colorado.edu, general questions can also be answered.

Loading... Loading...
You have selected an option that triggers this survey to end right now.
To save your responses and end the survey, click the 'End Survey' button below. If you have selected the wrong option by accident and/or wish to return to the survey, click the 'Return and Edit Response' button.